Session 9: Tumor Evolution

Topics Covered

• Introduction to tumor evolution
• Tumor heterogeneity
• Cancer subclonal reconstruction from DNA sequencing
• Timing somatic events in the evolution of cancer
• Evolutionary dynamics of extrachromosomal DNA (ecDNA) in human cancers

Practical

• Introduction to NGSpurity for calculating tumor purity and ploidy
  • Initial tumor profile solution for somatic copy number alterations
  • Refitting Solution for somatic copy number alterations
• Palimpsest R package
  • Clonality plots and Cancer cell fraction (CCF)
  • Temporal evolution of mutational signatures
  • Copy number alterations
  • Natural history of tumors

Supplemental

• Multi-Region Sequencing Analysis with REVOLVER